Studying Biomolecular Interactions: A Hybrid Approach

Characterization of Biomolecular Interactions Using a Multivariate Approach

Affinity capillary electrophoresis: a physical-organic tool for studying interactions in biomolecular recognition.

Affinity capillary electrophoresis (ACE) is a technique that is used to measure the binding affinity of receptors to neutral and charged ligands. ACE experiments are based on differences in the values of electrophoretic mobility of free and bound receptor. Scatchard analysis of the fraction of bound receptor, at equilibrium, as a function of the concentration of free ligand yields the dissociat...

Modeling of biomolecular interactions

In the beginning of the 21st century, one of the main goals of society is to increase the quality of life and maintaining the full performance and physical activity throughout the whole lifetime. This leads to extremely rapid development of all areas of human knowledge related to the study of living matter and to fast application of the obtained results for society benefit. Special attention is...

A robust numerical algorithm for studying biomolecular transport processes.

We present a numerical algorithm that is well suited for the study of biomolecular transport processes. In the algorithm a continuous Markov process is discretized as a jump process and the jump rates are derived from local solutions of the continuous system. Consequently, the algorithm has two advantages over standard numerical methods: (1) it preserves detailed balance for equilibrium process...

Electrostatic Interactions in Biomolecular Systems

Electrostatic interactions are of fundamental importance in determining the structure, dynamics, and function of biomolecules. In particular, they play a key role in protein folding and stability, pH-induced conformational changes, recognition of substrates by receptors, enzymatic catalysis, and in the formation of polysaccharide-based gels. However, due to their magnitude and long-range nature...